Now, with the advent of the new OpAns’ patented amasse collection device, DBS can be used to streamline QUANTITATIVE bio-fluids testing (e.g., capillary blood) using state-of-the-art analytical techniques like mass spectrometry with chromatographic separations.
Avantages include reduction in pain and anxiety associated with venipuncture; reduction in processing, handling and shipping requirements resulting in reduction in costs.
Steroid hormones were quantified using high-performance liquid chromatography followed by tandem mass spectrometry (HPLC-MS/MS).  Detection limits varied depending upon analyte.   Media samples were extracted using methyl tert-butyl ether (MTBE); derivatization with dansyl chloride was required for estrone and estradiol.
Test articles were incubated in Liver S9 fractions at 37 °C.   Samples were pulled from the incubation mixture at specified time points (0 to 90 minutes) and added to a solution of 1% ammonium hydroxide in N-propyl alcohol.   The amount of test article in each sample was quantified by HPLC-MS/MS.
Trimethoprim (TMP) and sulfamethoxazole (SMX) concentrations were quantified using validated liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assays.   The TMP validation range for the assay was 25 to 50,000 ng/mL.   The SMX validation range for the assay was 250 to 500,000 ng/mL.
Federal Drug Administration's Good Laboratory Practices (GLPs) govern conduct of nonclinical laboratory safety studies that support/are intended to support applications for research or marketing permits for drug products, part 11 of Title 21 CFR - Electronic Records and Electronic Signatures, and Bioanalytical Method Validation Guidance for Industry
Environmental Protection Agency's Good Laboratory Practices (GLPs) govern conduct of studies that support applications for research or marketing permits for pesticide products; Quality Management Plan (QMP) sets forth the requirements for an organization's quality management system to govern environmental programs