OpAns’ proprietary HPLC/MS/MS method for the determination of up to 14 Steroids in Plasma and H295R Cell Culture Medium has been successfully validated to FDA guideline standards and has been used to support numerous EPA and FDA regulated studies. The method accurately measures, from a 200 µL specimen aliquot, the following steroids:

Aldosterone (free alcohol)
Androstenedione (non-salt form)
Corticosterone (free alcohol)
Cortisol (free alcohol)
Cortisone (free alcohol)
Dehydroepiandrosterone (DHEA) (free alcohol)
Deoxycorticosterone (DOC) (free alcohol)
11-Deoxycortisol (free alcohol)
Dihydrotestosterone (DHT) (free alcohol)
Estradiol (free alcohol)
Estrone (free alcohol)
17α‑OH‑Progesterone (free alcohol)
Progesterone (non-salt form)
Testosterone (free alcohol)

Studies supported include:

Chemical Industry EPA Safety studies
Pharmaceutical R&D Rat Toxicity studies
Pharmaceutical R&D Monkey Toxicity studies